Fig. 1. 7-Ketocholesterol activates RBC-NOX through PKCz-RacGTPase signaling pathway. Effect of NOX inhibitor DPI or Rac GTP specific inhibitor NSC23766 on the oxysterol-induced RONS formation (A), PS-externalization (B), and PKCz translocation to membrane (C). RBCs were pre-incubated for 1h with inhibitor or vehicle before adding the oxysterol. RBCs were lysed after 1 h of incubation for Western blot detection of PKCz, while were left to incubate for 2 h or 24 h before the cytofluorimetric measure of DCF-associated MFI (mean fluorescence intensity) and AnnexinV-FITC associated cell fluorescence, respectively. Data are the means±SD of six (A and B) or three (C) separate experiments with a representative image. *Significantly different vs control (P<0.0001); § significantly different vs samples of the relevant group pre-incubated in the absence of inhibitor (P<0.0001) (one-way Anova associated with Bonferroni's post hoc test).